May i Use ShortCode in Template?

Have you tried using the do_shortcode() function?

EDIT

I’m not familiar with the TweetMeme shortcode, but here’s an example usage for putting a NextGen Gallery directly into a template file:

echo do_shortcode( '[slideshow id="1" w="603" h="270"]' );

Simply replace with the appropriate shortcode (and parameters) for TweetMeme.

Codex ref: do_shortcode()

EDIT 2

With TweetMeme, you have another option:

1. Change the Plugin “where” setting from “shortcode” to “manual”
2. Place the tweetmeme() function in your Theme template file where you want to output the TweetMeme button.