You may want to look at adding admin style and then targeting the menu:
function admin_style() {
wp_enqueue_style('admin-styles', get_template_directory_uri().'/admin.css');
}
add_action('admin_enqueue_scripts', 'admin_style');
This is just an example:
#menu-comments:before {
border-top: 1px solid red;
content: "";
display: block;
margin-top: 10px;
margin-bottom: 10px;
width: 100%;
}
This adds a red divider above the “comments” menu, but adjust this to your own requirements.