Usually: no.
When WordPress is running on apache with mod_rewrite enabled, it’ll use
# BEGIN WordPress
<IfModule mod_rewrite.c>
RewriteEngine On
RewriteBase /
RewriteRule ^index\.php$ - [L]
RewriteCond %{REQUEST_FILENAME} !-f
RewriteCond %{REQUEST_FILENAME} !-d
RewriteRule . /index.php [L]
</IfModule>
# END WordPress
To make sure that static files will NOT be served through WP.
A plugin might add its own rewrite rules to the .htaccess, but generally WP will not interfere with static files, those will be dealt with by apache directly.
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