Multiple arrays in post__not_in parameter

No, it doesn’t. You’d just merge the arrays:

'post__not_in' => array(
    array_merge( get_option( 'sticky_posts' ), array( 1, 2, 3 ) ),
)

And just because someone else will mention it if I don’t: post__not_in has terrible performance and you’d be better off finding an alternate solution.

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