IIS 7 also creates “IUSR” as default user to access files via IIS. So make user IUSR has read access to files/folders.
How to check if IUSR has read Access?
Right Click -> Folder -> Properties -> Security Tab
See if IUSR is in Group or user names list, If No.
Click Edit -> Add -> Advanced -> Find Now -> Select IUSR and click OK four times
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