If you want your WP site to function completely in subfolder, just set it up there as usual (including .htaccess) and do not add any directives to the root.
Setup of .htaccess in root, while WP is in subfolder, is only necessary if you want to have core files (and so admin area) there, but have site itself be root-based. As per Giving WordPress Its Own Directory instructions in Codex.
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