Problem using is_single() to enqueue script from functions.php

This could be one of a few different issues.

1. Wrong Hook / Not Hooked

You need to make sure that wp_enqueue_script is hooked to wp_enqueue_scripts. Otherwise, the call can get fired too late or (possibly in this case?) too early?

Try this (also with your php cleaned up a bit:

function wpse53364() {
    if( !is_admin() && is_single() ) {
        wp_register_script( 'jquery-scroll', get_bloginfo('stylesheet_directory').'/libs/jquery.jscrollpane.min.js' );
        wp_enqueue_script( 'jquery-scroll' );
    }
}
add_action( 'wp_enqueue_scripts', 'wpse53364' );

2. Incorrect Template Use

It’s unclear from your question, but if you’re using single.php for your “custom single posts template,” you shouldn’t be using that “Template Name:…” header. The WordPress template hierarchy will automatically know which file to use.

3. Wrong Conditional Tag

The fact that you’re using a custom page template makes me wonder whether you’re trying to use this script to enqueue the script on PAGES as opposed to POSTS. If that’s the case, you need to replace is_single() with is_page() or is_singular( 'page' )

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