Go to your WP-ADMIN–>Settings–>Permalink and use the permalink structure change there, if it generate any .htaccess file copy the content and update your .htaccess file.
Or Check if your hosting mod_rewrite is enable by creating a file phpinfo.php with content,
<?php phpinfo();?>
Upload this file and browse via Browser. So you know which modules are enabled. You need mod_rewrite enable to remove index.php from URL.
More details Check this link
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