External app using wp-load on subdomain

I think that’s getting triggered by wp-load being unable to identify which blog you’re on at load time. I’d try

define( 'SITE_ID_CURRENT_SITE', 1 );
define( 'BLOG_ID_CURRENT_SITE', 1 );

before you include wp-load, looking up the correct IDs for domain.com/app/ from your wp_blogs table.

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