How do I chop/slice/trim off last character in string using Javascript?

You can use the substring function:

let str = "12345.00";
str = str.substring(0, str.length - 1);
console.log(str);

This is the accepted answer, but as per the conversations below, the slice syntax is much clearer:

let str = "12345.00";
str = str.slice(0, -1); 
console.log(str);