OSError: [WinError 193] %1 is not a valid Win32 application

The error is pretty clear. The file hello.py is not an executable file. You need to specify the executable:

subprocess.call(['python.exe', 'hello.py', 'htmlfilename.htm'])

You’ll need python.exe to be visible on the search path, or you could pass the full path to the executable file that is running the calling script:

import sys
subprocess.call([sys.executable, 'hello.py', 'htmlfilename.htm'])

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