Python Subprocess: Too Many Open Files

I guess the problem was due to the fact that I was processing an open file with subprocess:

cmd = "enerCHARMM.pl -par param=x,xtop=topology_modified.rtf,xpar=lipid27_modified.par,nobuildall -out vdwaals {0}".format(cmtup[1])
p = subprocess.Popen(cmd, stdout=subprocess.PIPE, shell=True)

Here the cmd variable contain the name of a file that has just been created but not closed. Then the subprocess.Popen calls a system command on that file. After doing this for many times, the program crashed with that error message.

So the message I learned from this is

Close the file you have created, then process it

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