The length of the test_groups vector is 4 while the number of rows of subset is 5.
Below I set to 5 the number of rows of subset and the adonis command works nicely.
library(vegan) subset <- matrix(c(0,0.000666667,0.001333333,0.000333333,0.000333333,0.019833333,0.007666667,0.014666667,0.0005,0.022833333,0.016833333,0.018166667,0.000666667,0.009666667,0.008833333,0.009166667,0.001333333,0.018666667,0.0295,0.031833333),ncol=5) test_groups <- c(1,1,2,2) adonis(subset~test_groups)
Here is the result.
'nperm' >= set of all permutations: complete enumeration.
Set of permutations < 'minperm'. Generating entire set.
Call:
adonis(formula = subset ~ test_groups)
Permutation: free
Number of permutations: 23
Terms added sequentially (first to last)
Df SumsOfSqs MeanSqs F.Model R2 Pr(>F)
test_groups 1 0.22804 0.22804 1.0592 0.34624 0.3333
Residuals 2 0.43059 0.21530 0.65376
Total 3 0.65864 1.00000