How to create a non-responsive WordPress Theme Using Genesis Framework?

You can remove all the CSS code under the Media Queries section of any child theme built on Genesis. This code is generally located at the end of the style.css file.

However, WordPress also includes responsiveness built in so its not really possible to totally remove everything unless you go to a lot of trouble.

If you remove all the Media Queries, you will find your site will not look good when re-sized so its not a good idea to remove any CSS.