require one tag for each post

Have fun.

This works for any post type (including customs) that supports the ‘post_tag’ taxonomy. 99.9% of the time, it will apply only to ‘post’ post type. But we make compatible stuff 😉

/*
Plugin Name: Single Postag
Description: Enforces use of single 'post_tag' taxonomy on select posts.
Author: EarnestoDev
Version: 1.0.0
Author URI: http://www.earnestodev.com/
*/

// Prepare to save new post_tag
function postag_wp_insert_post($post_ID, $post){
    // Don't update if field value was not posted
    if(!isset($_POST['single_postag'])) return;
    // Fix WP slashing madness (I hate this joke)
    $postag = stripslashes(trim($_POST['single_postag']));
    $postag = $postag ? array(trim($postag)) : array();
    // Set object terms, don't append
    wp_set_object_terms($post_ID, $postag, 'post_tag', false);
}
add_action('wp_insert_post', 'postag_wp_insert_post', 10, 2); // 2 args required

// The metaxbox is here
function postag_meta_box($post, $box){
    // Get the tags and only keep names to weld
    $tags = wp_get_post_tags($post->ID);
    // Loop through as references for quick value reassignment
    foreach($tags as &$tag) $tag = $tag->name;
    // If multiple tags are added outside this widget, they are combined on save.
    echo '<p><input type="text" class="widefat" name="single_postag" value="',
        esc_attr(implode(', ', $tags)), '" /></p>';
    // Pimp description so your users can understand this
    echo '<p class="description">', 'Enter a single Tag.', '</p>';
}

// And now hijack the metaxbox
function postag_add_meta_boxes($post_type, $post){
    // Discard default 'post_tag' metabox (Important)
    remove_meta_box('tagsdiv-post_tag', $post_type, 'side');
    // Only add the new metabox if post_type supports 'post_tag' taxonomy
    if(!is_object_in_taxonomy($post_type, 'post_tag')) return;
    // Create a new 'tagsdiv-postag' metabox (you can't reuse the old MB name)
    add_meta_box('tagsdiv-postag', __('Tag'), 'postag_meta_box',
        $post_type, 'side', 'core');
}
add_action('add_meta_boxes', 'postag_add_meta_boxes', 10, 2); // 2 args required

Tested code. PHP 5.3+ Closures used. Convert code yourself to PHP 5.2 compatible variant if you need.

Regards.