If you choose subdomains, then you will have to configure wildcard subdomains on your server. That is pretty much going to be the end of your current subdomains. Well, not entirely. If you configure sub1.domain.at and sub2.domain.at before *.domain.at then sub1 and sub2 should probably still work. However, you cannot WordPress and another system running them at the same time. (Again, well, maybe but it will be very tricky – I do not recommend it.)
wpbeginner.com has a nice guide to setting up multisite. But you pretty much only need to find wp-config.php and add this:
define( 'WP_ALLOW_MULTISITE', true );
Then go Tools > Network Setup
and configure stuff. If you skipped enabling wildcard domains then you can pre-make your new subdomains in the WordPress admin area but all sub-sites will not work. When you are ready to switch over, enable wildcard subdomains and you are now switched to WordPress multisite.
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